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Apr 18, 2024
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BIO 5700 - Biotechnology: Cell Culture and Cloning
4 Credit(s) Hours: 6 hrs. /2 hrs. lecture, 1 hr. recitation, 3 hrs. laboratory
Biological principles underlying animal and plant tissue culture and cloning techniques are taught using current cell culture research publications. The formation and maintenance of primary and continuous culture, monolayer and suspension cultures, cell separation techniques, and cell cloning studies are conducted. Cell morphology in vitro, callus formation, cytochemistry, immunocytochemistry, autoradiography, chromosome spread preparation and karyotyping, western blotting and enzyme-linked immunoabsorbance assays are performed and analyzed. Photomicroscopy and independent research projects on the initiation and maintenance of primary cell cultures from such sources as chick embryos are also performed.
Prerequisite(s): BIO 1400 and CHM 1100 or Department Permission
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